Cold stress affects rice crop at seedling stage leading to poor seedling establishment, which accounts for the productivity losses of up to $30 million per annum. Once in 4 years rice crop also suffers from cold damage at reproductive stage which accounts to up to $120 million loss. Previous RIRDC funded projects revealed that external application of Osmoprotectants and Gibberellic Acid (GA) played a pivotal role in increasing rice cold tolerance. The results of this project revealed that there are genetic differences in rice for spermine (osmoprotectant) – and GA-accumulating ability associated with cold tolerance. This project also demonstrates that cultivars with higher cold tolerance at the seedling establishment stage will also have higher cold tolerance at reproductive stage. These findings are highly useful for the agronomists and plant breeders involved in developing cold tolerant rice cultivars.