National Honey Bee Breeding Strategy 2024-2029
In all livestock industries, genetic selection has been used to increase animal productivity and, by extension, enterprise profitability. The Australian honey bee industry is no...
HONEY BEE & POLLINATION
In-field validation of the newly developed AFB-LAMP assay
4 pages
Published: 28 Mar 2023
Author(s): Aidan Whitby
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Paenibacillus larvae, the bacterium responsible for American foulbrood (AFB), is a serious economic threat to apiaries. This bacterial infection can infect both strong and weak colonies, and is challenging to manage due to highly resistant spores that can remain viable for more than 50 years. Therefore, effective beekeeping practices are necessary to prevent the spread of AFB between hives.
A rapid and cost-effective diagnostic tool could significantly enhance management of AFB. Knowing this, the research team behind this project developed a field-deployable assay to detect P. larvae based on loop-mediated isothermal amplification (LAMP) technology, called AFB-LAMP. The assay’s diagnostic sensitivity (98.41%) and specificity (100%) were validated in the field, suggesting its suitability as a point-of-care assay. This rapid and affordable diagnostic tool could help apiarists protect their colonies from this devastating disease.
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