Expansion and refinement of a molecular typing system for Salmonella

SA Pathology trading as the Institute of Medical & Veterinary Science (IMVS)

  • Project code: PRJ-000751

  • Project stage: Closed

  • Project start date: Saturday, July 1, 2006

  • Project completion date: Thursday, October 29, 2009

  • National Priority: CME-Priority 4-Ensuring food safety of Australian chicken meat


The conventional and molecular typing services will continue to accumulate valuable data by monitoring the distribution of Salmonella serovars in chickens. This will contribute to food safety and public health objectives.
Provide a high resolution molecular based technique for the discrimination of within other important Salmonella such as Enteriditis and Infantis.


Chicken Meat

Research Organisation

SA Pathology trading as the Institute of Medical & Veterinary Science (IMVS)

Objective Summary

A free (up to 6 000 tests per annum) and discreet conventional and where appropriate molecular based typing service would continue to be offered to the industry. To further develop the bacteriophage-based molecular and MLVA typing systems developedin project IMV-5A and expand these typing systems into other serovars of interest to the chicken meat industry. To investigate improved turnaround times for MAPLT and MLVA systems by use of real time PCR multiplexing and to determine the minimal number of primer sets required yielding maximum separation in the most isolates.