Validation and kit development of LAMP test for C. psittaci and EHV1

The University of Queensland

  • Project code: PRO-017441

  • Project stage: Closed

  • Project start date: Tuesday, August 1, 2023

  • Project completion date: Thursday, August 1, 2024

  • National Priority: HOR-Thoroughbred diseases and parasites

Summary

The Agrifutures Thoroughbred Horses Program has previously invested in project PRJ-011628 – Diagnostic investigations into Chlamydia psittaci pathology in equine abortion, which developed Loop-mediated isothermal amplification (LAMP) tests for Chlamydia psittaci and Equine herpesvirus-1 (EHV1).  This project aims to validate and develop these tests to create an easy and efficient test kit to diagnose C. psittaci and EHV1 in thoroughbred horse foetal post-mortems. This project will undertake further validation of C. psittaci and EHV1 LAMP tests developed as part of project PRJ-011628 and translate this process into an easy-to-use test kit commercially available to stakeholders and adopted across the on-farm vets and veterinary clinics. 

Program

Thoroughbred Horses

Research Organisation

The University of Queensland

Objective Summary

  • Further development of the C. psittaci and EHV-1 LAMP test developed in PRJ-011628 – Diagnostic investigations into Chlamydia psittaci pathology in equine abortion to include assay quality assurance methodology.
  • Develop an easy to use prototype diagnostic test kit for multiplex LAMP Chlamydia psittaci/internal control and EHV-1/internal control deployed on the Genie III.