Development of Improved Molecular Detection Methods for Bacillus cereus Toxins

  • 68 pages

  • Published: 2 Feb 2006

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An important objective of this project was a study of collection of isolates of the bacterium Bacillus
cereus isolated in Australia to determine whether they are capable of producing toxins associated
with food poisoning. A large collection of isolates was made including bacteria isolated from food
and bacteria that had been isolated from food poisoning cases. In addition an extensive international
collection of samples of bacteria with a known profile of toxin production was imported.
As the emetic toxin has been associated with rice-based foods, the collection included 18 isolates
capable of producing the emetic toxin. The collection of Bacillus cereus isolates was supplemented
with several closely related Bacillus species as well as DNA extracted from the anthrax organism,
Bacillus anthracis.