Development and validation of Campylobacter microarrays for virulence detec

Royal Melbourne Institute of Technology

  • Project code: PRJ-000764

  • Project stage: Closed

  • Project start date: Tuesday, April 30, 2002

  • Project completion date: Sunday, July 30, 2006

  • National Priority: CME-Priority 1-Improving environmental sustainability outcomes

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Program

Chicken Meat

Research Organisation

Royal Melbourne Institute of Technology

Objective Summary

– To utilise existing knowledge on Campylobacter spp. to select the most appropriate virulence factors for use in the development of a Campylobacter microarray. – To design and select appropriate oligonucleotides for use in a Campylobacter array throughthe use of bacterial genomic techniques and alignment of gene sequences from known virulence factors to the Campylobacter genome. – To investigate the design of microchips for the immobilisation of oligonucleotides and hybridsation conditions for simultaneous detection of a variety of selected virulence factors.