Antimicrobial resistance is an emerging challenge for equine health, with implications for animal welfare, treatment effectiveness and potential human exposure. This project analysed ten years of equine bacterial culture and susceptibility data from different regions and practice types in Australia to better understand patterns of antimicrobial resistance. The research also evaluated newer diagnostic technologies, including MALDITOF mass spectrometry and minimum inhibitory concentration testing, to improve the speed and accuracy of bacterial identification and treatment selection. The findings support more informed, targeted antimicrobial use and contribute to the prudent management of antimicrobials within the Thoroughbred industry.